GENETIC ENGINEERING AND BIOTECHNOLOGY: (TOPIC)- REPORTER GENE ASSAY

 GENETIC ENGINEERING AND   BIOTECHNOLOGY

 REPORTER GENE ASSAY

In a reporter gene assay system a chimeric DNA contrast is designed with a gene that codes for a enzyme before the promoter of a gene of interest. the reporters contrast contains an enzyme for easy read-out and eukaryote promoter

LUCIFERASE REPORTER SYSTEM

It is an enzyme found in the abdomen of firefly photinuspyralis. This enzyme uses D-luciferin as a substrate to form oxyluciferin. The luciferin is converted into luciferin adenylate in the presence of ATP, Mg2+, by cleaving pyrophosphate (PPi) and transferring AMP to luciferin. Luciferin adenylate undergoes oxidative decarboxylation and forms oxyluciferin with the emission of light.